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Achat meloxidyl -CoA transferase), the major enzyme of fatty acid synthesis (14). To ensure that our results can be translated into practice, we will explore the use of pharmacological agents that have been shown to decrease the levels of lipogenic enzymes, such as FAS, FASG, and FASP, in the liver of healthy adults (16) and in obese patients (17). addition, we will explore whether dietary manipulations that increase energy intake or decrease food consumption may affect the levels of lipogenic enzymes (eg, increasing the fat content of a meal or diet). Finally, it will be of interest to examine the consequences of manipulating levels lipogenic enzymes for insulin-stimulated fatty acid synthesis. Materials and Methods Experimental design The first study design involved 3 subjects (M1, M2, best drugstore bb creams for pale skin and M3) each with 2 h of fasting before the initiation second study design (Figure 1A). Each subject underwent a 4-h fast followed by 1.5 h of glucose tolerance testing. Blood samples were collected at 6 h, 24 and 48 h for biochemical measurements, and adipose tissue biopsies were collected at 20, 40, and 60 h for lipid analyses. The fasting time was increased in the subsequent study designs by using a 1-h fast, and there were no differences between the 2 fasting time periods for glucose, insulin, insulin-like growth factor I (IGF-I), triglycerides, triglycerides-to-HDL-cholesterol ratio, and levels of circulating insulin-like growth factor binding protein-4 in the fasting blood samples. FIGURE 1 View largeDownload slide Study design of this (n = 3). Subjects M1 (M3) and M2 (M1) completed 2 consecutive 8-h fast periods and one 4-h period, respectively. Subjects M3 (M2) and M1 were not included in the study because they did not have adipose tissue samples and were not available. FIGURE 1 View largeDownload slide Study design of this (n = 3). Subjects M1 (M3) and M2 (M1) completed 2 consecutive 8-h fast periods and one 4-h period, respectively. Subjects M3 (M2) and M1 were not included in the study because they did not have adipose tissue samples and were not available. FIGURE 1 View largeDownload slide Study design of this (n = 3). Subjects M1 (M3) and M2 (M1) completed 2 consecutive 8-h fast periods and one 4-h period, respectively. Subjects M3 (M2) and M1 were not included in the study because they did not have adipose tissue samples and were not available. FIGURE 2 View largeDownload slide Study design of the 3-d (n = 3). Subjects M1 (M)